Microcapsule-based biosensor containing catechol for the reagent-free inhibitive detection of benzoic acid by tyrosinase
نویسندگان
چکیده
A biosensor based on the release of enzyme substrate from its structure was developed for inhibitive detection benzoic acid. polyurethane support comprising two perforated microcapsules (800 ?m in diameter) filled with methylene blue as a model compound and covered conductive deposit multiwalled carbon nanotubes, continuously released this stored dye 24 h. An increase concentration 0.5–0.75 ?mol L-1 h-1 1.5–2 h-1, presence absence nanotube coating, respectively, demonstrated by UV–vis spectroscopy 2 mL UV cuvette. The same configuration catechol modified laponite clay coating containing tyrosinase enzyme. resulting exhibits constant cathodic current at ?0.155 V vs AgCl/Ag, due to reduction ortho-quinone produced enzymatically catechol. acid recorded decrease inhibiting action activity. Reagentless biosensors different deposited quantity (100, 200, 400 600 ?g) were investigated applied inhibitor. best performance obtained ?g-based configuration, namely limit 0.4 sensitivity 228 mA L mol?1. After inhibition process, recover 97–100% their activity towards catechol, confirming reversible
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ژورنال
عنوان ژورنال: Biosensors and Bioelectronics
سال: 2021
ISSN: ['0956-5663', '1873-4235']
DOI: https://doi.org/10.1016/j.bios.2021.113137